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Image Search Results
Journal: bioRxiv
Article Title: Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network
doi: 10.1101/2022.01.20.477122
Figure Lengend Snippet: a. CRISPR/Cas9 DNA editing tool was used to generate deletions (green box) in the highly conserved DBD from vxs1 (top) and vsx2 (bottom) TFs. Blue boxes represent gene exons, black boxes the location of sgRNAs used to guide Cas9 endonuclease and primers for screening are depicted as opposing arrowheads. b-d and f-h. Histological sections stained with nuclear marker DAPI and phalloidin-Alexa488 for actin filaments from WT (b-d, n≥8) and vsx KO central retinas (f-h, n≥10) at 48hpf (b, f), 72hpf (c, g) and 6dpf (d, h). e, i. Head dorsal view from 6dpf WT (e) and vsx KO (i) larvae with insets showing their pigmentation pattern (white arrowhead). ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer, hpf: hours post-fertilization, dpf: days post-fertilization. Scale bar in b-d and f-h: 50μm, scale bar in e and i: 500μm.
Article Snippet: To target individual vsx genes, a solution containing two sgRNAs (40 ng/μL each) and
Techniques: CRISPR, Staining, Marker
Journal: bioRxiv
Article Title: Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network
doi: 10.1101/2022.01.20.477122
Figure Lengend Snippet: a. CRISPR/Cas9 was used to eliminate (green box) the DBD from vxs1 (top) and vsx2.1 (bottom) TFs in medaka. Blue boxes represent exons, black boxes the location of sgRNAs used and primers for screening are depicted as opposing arrowheads. b-e. Histological sections from WT (b, d, n=4) and vsx KO central retinas (c, e, n=5) at 12dpf. ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer, hpf: hours post-fertilization, dpf: days post-fertilization. Scale bar b-c: 50μm, d-e: 20 μm.
Article Snippet: To target individual vsx genes, a solution containing two sgRNAs (40 ng/μL each) and
Techniques: CRISPR